lacz genes การใช้

• Consequently, all clone of cells derived from GSC are marked with a functional lacZ gene.
• The lacZ gene was chosen because it produces galactosidase, an enzyme that is easy to detect in tissue because it turns blue when treated with the proper chemicals.
• G207 was constructed as a second-generation vector from HSV-1 laboratory strain F, with ICP34.5 deleted and the ICP6 gene inactivated by insertion of the E . coli LacZ gene.
• According to their findings, it is possible to track male GSCs labeled with lacZ gene in " Drosophila " model by inducing recombination with heat shock and observe the decrease in GSC number with aging.
• The blue / white screening method relies on the principle of ?-complementation of the ?-galactosidase gene, where a fragment of the lacZ gene ( lacZ? ) in the plasmid can complement another mutant lacZ gene ( lacZ?M15 ) in the cell.
• The blue / white screening method relies on the principle of ?-complementation of the ?-galactosidase gene, where a fragment of the lacZ gene ( lacZ? ) in the plasmid can complement another mutant lacZ gene ( lacZ?M15 ) in the cell.
• In order to mark GSCs with lacZ gene, flip recombinase ( Flp )-mediated recombination is used to combine a ubiquitously active tubulin promoter followed by an FRT ( flip recombinase target ) site with a promotorless lacZ ORF ( open reading frame ) preceded by an FRT site.
• First in laboratory tissue cultures and then in shaved mice, the researchers demonstrated that liposomes containing the so-called lacZ gene deposited the gene in the hair follicles near the base of the hair shaft . Without a liposome jacket, lacZ genes applied to skin tissue did not appear in the follicles.
• First in laboratory tissue cultures and then in shaved mice, the researchers demonstrated that liposomes containing the so-called lacZ gene deposited the gene in the hair follicles near the base of the hair shaft . Without a liposome jacket, lacZ genes applied to skin tissue did not appear in the follicles.